Preanalytical, Analytical, and Computational Factors Affect Homeostasis Model Assessment Estimates

Preanalytical, Analytical, and Computational Factors Affect Homeostasis Model Assessment Estimates

OBJECTIVE We investigated how beta-cell function and insulin sensitivity or resistance are affected by the type of blood sample collected or choice of insulin assay and homeostatis model assessment (HOMA) calculator (http://www.dtu.ox.ac.uk). RESEARCH DESIGN AND METHODS Insulin was measured using 11 different assays in serum and 1 assay in heparinized plasma. Fasting subjects with normoglycem...

The preanalytical and analytical factors responsible for false-positive cardiac troponins

Analysis of circulating microRNA: preanalytical and analytical challenges.

BACKGROUND There is great interest in circulating microRNAs (miRNAs) as disease biomarkers. Translating promising miRNAs into validated clinical tests requires the characterization of many preanalytical and analytical parameters. METHODS miRNAs were extracted from serum and plasma samples of healthy volunteers, and miRNAs known to be present in serum and plasma (miR-15b, miR-16, miR-24, and m...

Assessment and recommendations on factors contributing to preanalytical variability of urinary pyridinoline and deoxypyridinoline.

BACKGROUND Pyridinoline (PYD) and deoxypyridinoline (DPD) are two of the most extensively characterized biochemical bone markers, but the interpretation of results is hampered by biologic and other preanalytical variability. We reviewed factors contributing to preanalytical variation of pyridinium cross-links in urine. METHODS We searched four databases for English-language reports on PYD and...

Lead – a Preanalytical/analytical Variable in Clinical Chemistry

Lead is one of the most studied clinically important metals due its high toxicity and a high number of workers exposed to it. The interest toward Pb is elevated by the fact that children are especially susceptible to lead poisoning. Research regarding lead poisoning requires a complex, multi-disciplinary (clinical medical and clinical chemical) approach. Monitoring human exposure to lead (intak...